ABSTRACT
Objective To investigate the value of combined detection of viral capsid antibody(VCA -IgA), early antigen antibody(EA -IgG),and Rta protein antibody IgG(Rta -IgG)in the diagnosis of EB virus associated nasopharyngeal carcinoma(NPC).Methods A total of 47 nasopharynx cancer patients,45 patients with benign rhinitis and 45 healthy controls were recruited.The serum levels of VCA -IgA,EA -IgA and Rta -IgG were tested by enzyme -linked immunosorbent assay (ELISA).Specificity and sensitivity of the indicators alone and combined detection were compared using the clinical diagnosis as the gold standard.Results The expression levels of serum VCA -IgA,EA -IgA and Rta -IgG in the rhinitis group were (0.82 ±0.25),(0.74 ±0.13),(0.89 ±0.27),the levels in the NPC group were (2.16 ±0.39),(1.26 ±0.24),(3.95 ±0.76),and the levels in the healthy control group were (0.65 ±0.14),(0.51 ±0.11),(0.41 ±0.16)respectively.The levels of VCA -IgA,EA -IgA and Rta-IgG in the rhinitis group,NPC group and healthy group were significantly different (F =400.065,232.803, 740.215,P =0.000,0.000,0.000).The levels of VCA -IgA,EA -IgA and Rta -IgG in the NPC patients with different TNMstages were significantly different(F =195.679,30.878,38.561,P =0.000,0.000,0.000),and the trend of each antibody was increased with the severity of the disease.The sensitivity of VCA -IgA was the highest (85.11%),and the specificity of EA -IgA was the highest(95.56%).The sensitivity of the combined assay was 95.74%,which was higher than that of the other three combinations.Conclusion The combination of VCA -IgA, EA -IgA and Rta -IgG can reflect the expression of EBV -associated antigen to a greater extent,and it is superior to single or two combined detection in the diagnosis of EB -associated NPC,with a high clinical value.
ABSTRACT
Objective To explore the effects of traditional Chinese formula Danchaiheji on the differentiation of regula?tory dendritic cells (DCs) and the underlying mechanism. Methods The rat blood serums with or without the formula Dan?chaiheji were prepared. The peripheral blood mononuclear cells were separated from the peripheral venous blood of healthy donors. CD14+monocytes were isolated using CD14+magnetic beads and cultured for 5-7 days to obtain immature dendritic cells (imDCs). Then the cells was divided into control group and Danchaiheji containing rat serum group. Control group was divided into two subgroups (containing LPS and without LPS). Danchaiheji containing rat serum group was also divided into two subgroups (containing LPS and without LPS). The surface markers CD86, CD11b and HLA-DR of DCs were detected by flow cytometry. The level of IL-10 was determined by enzyme-linked immunosorbent assays (ELISA). The proliferation of al?logeneic T-cells was detected by flow cytometry and the expression level of indoleamine 2,3-dioxygenase (IDO) was deter?mined using quantitative real-time PCR. Results DCs treated with the formula Danchaiheji exhibited high CD11b and low CD86 and HLA-DR expression levels as well as promoted the secretion of IL-10. In addition, the drug could inhibit the pro?motion of DCs on the proliferation of T cells, which was associated with the up-regulation of IDO expression. Conclusion The traditional Chinese formula Danchaiheji can induce the differentiation of DCs into regulatory DCs and play a role in in?hibitory effect on immune function.
ABSTRACT
Dendritic cells (DCs) are potent antigen presenting cells with dual functions in immune response or im-mune tolerance. Regulatory dendritic cells (DCreg) have negative immunomodulation effects. They can inhibit T cell stimula-tory activity and induce immune tolerance. DCreg can be generated by the induction of drugs, cytokines and cell microenvi-ronment with different mechanisms. DCreg induced tolerance is of great clinical significance in organ transplantation and au-toimmune disease. Recently, several clinical trials have been conducted in type 1 diabetes and rheumatoid arthritis, with re-sults that emphasize the tolerance and safety of DCreg therapy. In this review, we will focus on the strategies for generating DCreg and the clinical application of immune tolerance.